For first time treatment, it’s recommended to use continuously for 14 days. Thereafter, for maintenance just apply 2 – 3 bottles a week. One bottle of ampoule can last for a day and it’s advisable to finish the ampoule within the day. Take note that the ampoules must be store away from heat and sun. The best part of using the ampoule is that there’s no need to apply moisturiser or cream as it’s rich enough on its own.